essay格式：紫外线光谱

essay格式：紫外线光谱

essay格式：紫外线光谱

UV Visible spectroscopy can be used for the process of quantitative analysis of a large number of pharmaceutical substances. It is one of the most important techniques which is used for the purpose of analysis of the different pharmaceutical substances. It involves the process including the measurement of the amount of ultraviolet or the visible radiation. Thus measurement is done when ultraviolet or the visible radiation is absorbed in the solution. There are a large number of drugs which can be used either in the form of raw materials or used as a formulation (Kemp & William, 1991). The solution of the drugs is made and the measurement of absorbance at a specific wavelength is done. An example of the same is the quantitative analysis of the Diazepam tablet. This tablet can be analyzed by mixing 0.5 % of H2SO4 in methanol at a given wavelength having a value of around 284 nm. The instrument which is used for measurement of the ratio or the function of ratio or the intensity is known as the ultraviolet visible spectrophotometers.
While during the qualitative analysis, it can be easy to do the identification of the organic compounds with the help of UV Visible spectrometry. On the other hand the quantitative analysis is done in order to ensure the number of molecules which absorbs the radiation. The usefulness of this technique is that it is simple, specific and can be applicable to a large number of compounds.The fundamental law which can be used for governing this process is the Beer’s law and the Lambert’s law. According to this law, the process related to the absorbance for this solution is related to the concentration of some of the most important absorbing species which is there in the solution and the length of the path. It is used for the qualitative analysis of those compounds which absorbs the UV Radiation. Lambert’s law states that the overall value of intensity of a beam of a monochromatic radiation which is parallel to one another would decrease in an exponential manner (Nockemann et al., 2005). This decrease occurs when the beam passes through the medium which has a homogenous thickness.