本研究的目的是了解课程代写-SWELL1对细胞脂质代谢和肥胖的调节作用，我们将分析SWELL1对akt2介导的葡萄糖稳态调节和脂解途径。这将使肥胖中脂肪组织膨胀的膨胀调节成为可能。本研究的基本目的是分析瘦、胖和禁食小鼠体内的脂肪细胞。为达到这一目的，我们将在可诱导和控制的SWELL1 KO小鼠中探索瘦鼠和肥胖小鼠脂肪细胞肿胀1的基础和刺激作用。测定肥胖小鼠禁食后脂肪细胞的基底电流和受刺激的膨胀电流。第二个目的是了解SWELL1-PI3k-Akt信号在脂肪细胞中的作用机制。因此，我们将对WT和体内外SWELL1 KO脂肪细胞中胰岛素- pi3k – akt信号通路的特性进行研究。第二种方法是分析SWELL1活性是否会影响胰岛素- pi3k – akt信号通路。接下来将研究和确定SWELL1 c端富亮氨酸重复域(LRRD)，用于调控胰岛素- pi3k – akt信号通路。接下来有关课程代写-SWELL1对细胞脂质代谢和肥胖的调节作用分享给大家阅读。
The fundamental aims of this research in SWELL1 are to analyze the adipocyctes from lean, obese and fasting mice. To achieve this basal and stimulate SWELL1 in adipocyte of lean and obese mice in controlled and inducible SWELL1 KO mice will be probed. There will be measurement of the basal and the stimulated SWELL1 current in adiopocyte of obese mice after the periods of fasting will be determines. The second aim is to understand the mechanism of SWELL1-PI3k-Akt signaling in adipocycte will be probed. For this, there will be characterization of the Insulin-PI3K-AKT signaling in the WT and SWELL1 KO adipocytes in vitro and in vivo will be probed. Second approach is analyzing if the SWELL1 actition can impact the insulin-PI3K-AKT signaling. From there will be investigation and determination of the SWELL1 C-terminal leucine-rich repeat domain (LRRD) for SWELL1 regulation of insulin-PI3K-AKT signaling. The third aim of this research is to understand the regulatory effects of the SWELL1 in lipid metabolism and adiposity of obesity in the cells. There will be analysis of the SWELL1 regulation of AKT2-mediated glucose homeostatis and lipolysis pathways. This would enable in the SWELL1 regulation of the adipose tissue expansion in obesity.
To understand about these mechanisms there has been analysis done on TRPM7, TRPV4, TRPC6 and Piezo-1/ Piezo 2. In the prior research that was undertaken by us, it was proven that TRPV4 is a cell-autonomous mediator that functioned as oxidative metabolism, inflammation, energy homeostasis in the adipocyte tissues. We had previously speculated that TRPV4 can represent the swell/stretch-activated channel in the adipocyte. The prior research that was undertaken by using the patch-clamp electrophysiology revealed that the mechanism of the swell/stretch-activated ionic current. This was found to resemble the volume-regulated anion current (VRAC) and was assumed to be responsible for the cellular volume regulation.
Recent literature reports that molecular identity of this swell-activated current should be LRRC8a or Leucine Rich Repeat Containing 8a – renamed SWELL1 by Qiu and the research team.